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ERX2067695: Illumina HiSeq 2500 sequencing; Transcriptome analysis of Triticum monococcum in response to cold stress (4 degrees C for 24 hours)
1 ILLUMINA (Illumina HiSeq 2500) run: 9.9M spots, 491.6M bases, 205.8Mb downloads

Design: Transcriptome analysis of Triticum monococcum in response to cold stress (4 degrees C for 24 hours)
Submitted by: Oregon State University
Study: Transcriptome analysis of Triticum monococcum in response to cold stress (4 degrees C for 24 hours)
PRJEB21284 • ERP023518 • All experiments • All runs
show Abstracthide Abstract
24-hour cold shock treatment applied to winter wheat T. monococcum ssp. aegilopoides (accession G3116) and the domesticated spring wheat T. monococcum ssp. monococcum (accession DV92). Tissues were harvested and homogenized using liquid nitrogen. RNA extraction was conducted using a modified chloroform extraction protocol (Plant Purification Reagent) followed by RNeasy Plant Mini Kit (50) for purification. Samples included: three G3116 control, three G3116 cold shock, three DV92 control, and three DV92 cold shock. Illumina samples were prepped using TruSeq RNA prep kit v.2 and sequenced on the Illumina Hiseq 2500 sequencing platform.
Sample: G3116 Control Replicate 1
SAMEA104124447 • ERS1788340 • All experiments • All runs
Organism: Triticum monococcum subsp. aegilopoides
Library:
Name: G3116 Control Replicate 1_s
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: SINGLE
Construction protocol: Plants were grown in normal greenhouse growth conditions Control samples were maintained at normal conditions at 23 degrees Celsius for 24 hours. Cold samples were exposed to temperature of 4 degrees Celsius for 24 hours. Total RNA was extracted from frozen plant tissue. Invitrogen Plant RNA Reagent, chloroform, isopropanol, and DNAse were used to obtain RNA. Qiagen RNA cleanup kit was used to remove any enzymes used in extraction. Single-end libraries were quantified by qPCR and prepared using an Illumina TruSeq RNA prep kit
Experiment attributes:
Experimental Factor: organism: Triticum monococcum subsp. aegilopoides
Experimental Factor: environmental stress: no stimulus
Experimental Factor: strain: accession G3116
Runs: 1 run, 9.9M spots, 491.6M bases, 205.8Mb
Run# of Spots# of BasesSizePublished
ERR20079309,910,339491.6M205.8Mb2020-01-09

ID:
9836666

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